KMID : 0360220110520111337
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Journal of the Korean Ophthalmological Society 2011 Volume.52 No. 11 p.1337 ~ p.1343
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Autophagy of Human Tenon¡¯s Capsule Fibroblasts Induced by Mitomycin-C
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Park Chan-Ny
Park Rae-Kil Ryu Seong-Kwang Lee Dong-Wook
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Abstract
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Purpose: The present study investigated whether an autophagic process is involved in the apoptotic death of human tenon¡¯s capsule fibroblasts (HTCFs) caused by mitomycin-C.
Methods: An autophagic phenotype was tested using fluorescence microscopy and flow cytometry with specific biological staining dyes including monodansylcadaverine and acridine orange and microtubule-associated protein 1 light chain 3 (LC3).
Results: Treatment with mitomycin-C (0.4 mg/ml) increased the acidic vesicular organelles of tenon¡¯s capsule fibroblasts in a time dependent manner. Mitomycin-C induced both LC3-II cleavage and beclin-1 expression. 3-MA, a pharmacological inhibitor of autophagy, inhibited the mitomycin-C induced increase of acidic vesicular organelleS.
Conclusions: Autophagy was induced with 0.4 mg/ml mitomycin-C in tenon¡¯s capsule fibroblasts. And, autophagic mechanisms may be involved in the early stage of apoptosis of fibroblasts.
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KEYWORD
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Autophagy, Fibroblast, Mitomycin-C
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